Wildlife   plays   an   important   role   in   the   transmissionof   pathogenic microorganisms to humans, pets and other wildlife, serving on many occasions as reservoir  and  vector  of  zoonotic  infections.  This  study aims to  observe  the presence  of  staphylococci  and Enterobacteriaceae of clinical  interest  in  griffon vulture  (Gyps  fulvus). Cloacal  swabs  from  birds both  in  wild  and  in  captivity were sampled. Eight staphylococcal  species and  six  enterobacteria  species were identified by classical biochemical tests or API,and their sensitivity profile to seventeen antibiotics was determined. The presence of these bacteria could involve a risk to Public Health,environment and wildlife. It would be necessary to study further the wildlife community to know the evolution of these infectious agents.